Coding

Part:BBa_K2617012:Design

Designed by: Changyu Li   Group: iGEM18_UESTC-China   (2018-09-30)


AtoB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 213


Design Notes

To get a good expression of this gene in E. coli, we did codon optimization before DNA synthesis.


Source

The source of the part will be its sequence which was retrieved from GenBank.

References